The Women's 500m time trial event at the 2010 South American Games was held on March 21.
| Gold | Silver | Bronze |
| Diana Maria Orrego Colombia |
Janny MIlena Zambrano Colombia |
Sumaia Ribeiro Brazil |
| Rank | Rider | 250m | Time |
|---|---|---|---|
| Diana Maria Orrego (COL) | 20.092 (1) | 35.441 | |
| Janny Milena Zambrano (COL) | 20.799 (2) | 36.831 | |
| Sumaia Ribeiro (BRA) | 21.170 (4) | 37.416 | |
| 4 | Marines Prada (VEN) | 21.059 (3) | 37.825 |
| 5 | Irene Aravena Cortes (CHI) | 21.334 (5) | 37.852 |
| 6 | Maira Barbosa (BRA) | 21.628 (6) | 38.852 |
| 7 | Daiana Almada (ARG) | 22.104 (9) | 39.427 |
| 8 | Caterine Priviley (ARG) | 22.057 (8) | 39.708 |
| 9 | Maria Paz Bravo (CHI) | 21.858 (7) | 39.718 |
| 10 | Valeria Bacilia Ruiloba (BOL) | 24.055 (10) | 42.242 |